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Present approaches to bloodstain analysis. Hb, hemoglobin; HLA, human leukocyte antigen; RFLP, restriction fragment length polymorphism; PCR, polymerase chain reaction; STRs, short tandem repeats; PM, polymarker.

Forensic Science RFLP DNA simulation activity

This is an excel file containing printable materials for use to simulate a very basic Restriction Fragment Length Polymorphism (RFLP) analysis of D...

RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLPs): Differences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths (DNA segments resulting from treatment with restriction enzymes).


This week on AZPI: Forensic DNA - Two different DNA tests exist, the RFLP and the PCR. Both are very accurate, but they’re conducted in different ways. The restriction fragment length polymorphism or RFLP (sometimes called DNA fingerprinting or profiling) is considered to be the more accurate of the two.

* PCR tubes - The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.


Placing a strip of eight PCR tubes, each containing a 100 μl reaction mixture, into the thermal cycler. (Polymerase chain reaction - Wikipedia, the free encyclopedia)