Thin-layer chromatography (TLC) is a very commonly used technique in synthetic chemistry for identifying compounds, determining their purity and following the progress of a reaction. It also permits the optimization of the solvent system for a given separation problem. In comparison with column chromatography, it only requires small quantities of the compound (~ng) and is much faster as well.
Cleaning capillary tubes with 35% H2O2 + muriatic acid mixture. From time to time we often run out of clean capillary tubes what are important if we would like to check our reactions and products purity with TLC (thin layer chromatography). The H2O2 and HCl mixture is a strongly oxidizing solution, what also dissolves gold (yes, not just aqua regia is suitable for this) and it also destroys everything what is organic, so it is perfect to clean glassware. Important note: elemental chlorine…
This page is an introduction to chromatography using thin layer chromatography as an example. Although if you are a beginner you may be more familiar with paper chromatography, thin layer chromatography is equally easy to describe and more straightforward to explain.
Column chromatography for beginners. First, look after an immobile phase (usually silica or alumina) what was tested before with the correct eluent (mixture of 2 or more organic solvent). If the mixture of compounds separate with this mixture on a TLC (thin layer chromatography) what is made from the same immobile phase it will hopefully work on a larger scale also.