The Urease Test Determines whether or not a microorganism can hydrolyze urea, a process that releases ammonia and produces a color change in pH indicators Urease hydrolyzes urea to form ammonia, water and CO2. Christensen urea agar is preferred In agar form, the slant is inoculated, not stabbed Phenol red is the pH indicator The resulting alkaline pH from hydrolysis of urea is indicated by a bright pink color
The blood urea nitrogen or BUN is a blood test that measures the amount of urea nitrogen in the bloodstream. It reflects how well your kidneys are. When a person is dehydrated, false results might be obtained as BUN levels increase when blood is concentrated. Elevated BUN levels indicate dehydration, excessive protein intake and impaired renal function. Low BUN levels indicate over hydration, liver damage and malnutrition.
Final lab, ex. 5. Urease test to determine if bacteria can breakdown urea using the enzyme urease. The color change in the phenol red comes from the breakdown of urea into ammonia...raising the pH to around 8+.
Creatinine is a waste product produced by muscle metabolism. Kidneys filter creatinine to be excreted through the urine and the renal function is reflected. Elevated levels indicate renal impairment. The opposite happens for urinary creatinine clearance; during renal impairment, urinary creatinine is extremely low. Measuring urinary creatinine clearance during unilateral kidney impairment is insignificant as the healthy kidney can still excrete creatinine through the urine.