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Isabelle of Portugal: Isabel de Aviz, Infanta de Portugal was born on 4 October 1503 at Lisbon, Portugal. She was the daughter of Manuel I de Aviz, Rei de Portugal and Maria de Castilla y Aragón, Infanta de Castilla.She married Karl V von Habsburg, Holy Roman Emperor, son of Felipe I von Habsburg, Rey de Castilla and Juana, Reina Juana de Castilla, on 11 March 1526 at Seville, Spain. She died on 1 May 1539 at age 35 at Toledo, Spain.