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Lady Jane Grey, Queen for 9 days. According to Edward VI's last will, she was due to succeed him on the throne, even though she didn't want to, believing that Mary, daughter of Henry the VIII, had more right. Mary was acclaimed queen in stead of her, and had her imprisoned and executed.
Lady Mary Grey, 1571 (attributed to Hans Eworth) (ca. 1520-1574) Chequers Estate, Buckinghamshire