The Roanoke Colony on Roanoke Island in present-day North Carolina was a late 16th-century attempt to establish a permanent English settlement in what later became the Virginia Colony. The final group of colonists disappeared during the Anglo-Spanish War, three years after the last shipment of supplies from England. The settlement is known as "The Lost Colony," and the fate of the colonists has never been determined.
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The New Island Behind Hispania by Sebastian Münster, 1540. This was the first separate map of the Americas. Note the dismembered human tied to a tree in South America near the text Canibali, and the city of Temistitan (or Tenochtitlan, now Mexico City). Ferdinand Magellan’s voyage is symbolized by a ship in the Pacific Ocean.