"Mary, Queen of Scots, age 9" I have never been quite convinced that this is Mary--Mary had a long, narrow, beaky nose (not unlike her cousin Elizabeth's) and this lady's nose is positively snub! If it's Mary, it certainly doesn't look like the Clouet portraits.
Mary Tudor (Bloody Mary) earned for having had 300 people burned at the stake (in three and 1/2 years) in her religious persecutions to restore the Roman Catholic Church's place in England. Mary was Elizabeth I's older sister. Mary was the daughter of King Henry VIII's first wife, Catherine of Aragon,
Mary Queen of Scots was one of the most fascinating and controversial monarchs of 16th century Europe. At one time, she claimed the crowns of four nations - Scotland, France, England and Ireland. Her physical beauty and kind heart were acknowledged even by her enemies. Yet she lacked the political skills to rule successfully in Scotland. Her second marriage was unpopular and ended in murder and scandal; her third was even less popular and ended in forced abdication in favor of her infant son...
Mary Queen of Scots 1542-1587 - (Mary Stuart or Mary I of Scotland) was queen regnant of Scotland. Mary spent most of her childhood in France whilst Scotland was ruled by regents, and in 1558, she married Francis, Dauphin of France. Vivacious, beautiful, and clever, Mary had a promising childhood. She was considered a pretty child and later, as a woman, strikingly attractive. She was a favourite with everyone, except Henry II's wife Catherine de' Medici.
Probably "Unknown English Lady, school of Larkin" c. 1580 and probably not Mary Queen of Scots. Comparison to the undoubted Mary of Clouet would seem to make the connection doubtful just on the basis of the subject's appearance.